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Addgene

pAAVS1 Insulator ObLiGaRe/EPB71
(Plasmid #90018)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 90018 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57
  • Backbone manufacturer
    GeneWiz
  • Backbone size w/o insert (bp) 2500
  • Total vector size (bp) 3700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MCS flanked by insulator and inverted ZFN binding sites
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1235

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The ZFN binding sequence is 5΄-atcctgtccctaggccacccactgtgggt-3΄.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAVS1 Insulator ObLiGaRe/EPB71 was a gift from Eric-Paul Bennett (Addgene plasmid # 90018 ; http://n2t.net/addgene:90018 ; RRID:Addgene_90018)

  • For your References section:

    Precise integration of inducible transcriptional elements (PrIITE) enables absolute control of gene expression. Pinto R, Hansen L, Hintze J, Almeida R, Larsen S, Coskun M, Davidsen J, Mitchelmore C, David L, Troelsen JT, Bennett EP. Nucleic Acids Res. 2017 May 4. doi: 10.1093/nar/gkx371. 10.1093/nar/gkx371 PubMed 28472465
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