pCDX2 ObLiGaRe Donor vector/EPB64
(Plasmid
#90017)
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PurposeDonor vector for ObLiGaRe/ZFN mediated targeting to CDX2 exon1 locus
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 90017 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC57
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Backbone manufacturerGeneWiz
- Total vector size (bp) 2500
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMCS flanked by inverted CDX2 ZFN binding sites
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SpeciesH. sapiens (human)
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Insert Size (bp)173
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Entrez GeneCDX2 (a.k.a. CDX-3, CDX2/AS, CDX3)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13for
- 3′ sequencing primer M13rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The sequence of the CDX2 ZFN binding sites is AACCGCCGTAGTCCGGGTAccgcagGGGGCTGACGAAGTT.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDX2 ObLiGaRe Donor vector/EPB64 was a gift from Eric-Paul Bennett (Addgene plasmid # 90017 ; http://n2t.net/addgene:90017 ; RRID:Addgene_90017) -
For your References section:
Precise integration of inducible transcriptional elements (PrIITE) enables absolute control of gene expression. Pinto R, Hansen L, Hintze J, Almeida R, Larsen S, Coskun M, Davidsen J, Mitchelmore C, David L, Troelsen JT, Bennett EP. Nucleic Acids Res. 2017 May 4. doi: 10.1093/nar/gkx371. 10.1093/nar/gkx371 PubMed 28472465