pSF3-Flag-FRB-Cbir_Myc-NBir-FKBP
(Plasmid #90008)

• Purpose: Split-BioID plasmid: Expresses C-terminally tagged FRB-CBir[257-321] and N-terminally tagged NBir[2-256]-FKBP
• Depositing Lab: Julien Béthune
• Publication: Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.1038/ncomms15690.

Backbone

• Vector backbone: pSF3
• Total vector size (bp): 8582
• Vector type: Mammalian Expression, Retroviral ; Flp/FRT
• Selectable markers: Hygromycin ; Ganciclovir (negative selection when making stable cell lines)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: NBirA*-linker-FKBP
• Insert Size (bp): 1194
• Promoter: CMV
• Tag / Fusion Protein:
  • Myc (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: GTGGTTTGTCCAAACTCATC

Gene/Insert 2

• Gene/Insert name: FRB-linker-CBirA*
• Insert Size (bp): 627
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: tatactttctagagaataggaac

Resource Information

• Supplemental Documents:
  • pSF3-FLAG-FRB-CBir_Myc-NBir-FKBP.gb
• A portion of this plasmid was derived from a plasmid made by: Addgene plasmids: pLXIN-Ap1g1-FKBP (#46946) and pEYFP-Mitotrap (#46942) were kind gifts from Margaret Robinson and pCDNA3.1-MycBioID (#35700) was a kind gift from Kyle Roux

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry