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Addgene

NGN3-T2A-NLS-tdTomato-ires-puro-BGHpA-PGK-puro Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid NGN3-T2A-NLS-tdTomato-ires-puro-BGHpA-PGK-puro with the following enzymes: BamHI-HF and AgeI-HF.

We predict the following banding pattern:

BamHI-HF - 9.8kb
AgeI-HF - 9.8kb
BamHI-HF + AgeI-HF - 5.9kb & 3.9kb

We see the expected result.