NGN3-T2A-NLS-tdTomato-ires-puro-BGHpA-PGK-puro Notes
Addgene Diagnostic Digest
This gel depicts the NEB 1kb ladder and a digestion of the plasmid NGN3-T2A-NLS-tdTomato-ires-puro-BGHpA-PGK-puro with the following enzymes: BamHI-HF and AgeI-HF.
We predict the following banding pattern:
BamHI-HF - 9.8kb
AgeI-HF - 9.8kb
BamHI-HF + AgeI-HF - 5.9kb & 3.9kb
We see the expected result.