pUC19-SOX2-T2A-2xNLS-tdTomato-F2A-Puro Notes
Addgene Diagnostic Digest
This gel depicts the NEB 1kb ladder and a digestion of the plasmid pUC19-SOX2-T2A-2xNLS-tdTomato-F2A-Puro with the following enzymes: ClaI and NotI-HF.
We predict the following pattern:
Cla I - 6.3kb
NotI-HF - 6.3kb
Cla I + NotI-HF - 5kb & 1.3kb
We see the expected result.
