pPtGG-1
(Plasmid
#89990)
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Purpose(Empty Backbone) pBR322-based entry vector for Gateway cloning
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 89990 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBR322
- Backbone size (bp) 3742
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Modifications to backboneDeletion of AmpR
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Vector typeGolden gate entry vector
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPtGG-1 was a gift from Philip Weyman (Addgene plasmid # 89990 ; http://n2t.net/addgene:89990 ; RRID:Addgene_89990) -
For your References section:
Episomal tools for RNAi in the diatom Phaeodactylum tricornutum. Bielinski VA, Bolt TM, Dupont CL, Weyman PD. PeerJ Preprints. 2017 5:e2907v1 10.7287/peerj.preprints.2907v1
Map uploaded by the depositor.
