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Purposeinducible CRISPR-ON system for controllable gene activation; this plasmid is used to insert dCas9-VPR casette in one allele of AAVS1 locus
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 89985 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneAAVS1 homologous recombineering donor plasmid
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namedCas9-VPR
- Promoter TRE-tight
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAVS1-idCas9-vpr was a gift from Jie Na (Addgene plasmid # 89985 ; http://n2t.net/addgene:89985 ; RRID:Addgene_89985) -
For your References section:
An inducible CRISPR-ON system for controllable gene activation in human pluripotent stem cells. Guo J, Ma D, Huang R, Ming J, Ye M, Kee K, Xie Z, Na J. Protein Cell. 2017 Jan 23. doi: 10.1007/s13238-016-0360-8. 10.1007/s13238-016-0360-8 [pii] PubMed 28116670
