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Addgene

pRS314-prp16-302
(Plasmid #89974)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89974 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS314
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PRP16
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    prp16-302 allele (R456K and G691R)
  • Entrez Gene
    PRP16 (a.k.a. YKR086W, PRP23, RNA16)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS314-prp16-302 was a gift from Christine Guthrie (Addgene plasmid # 89974 ; http://n2t.net/addgene:89974 ; RRID:Addgene_89974)

  • For your References section:

    Prp8 retinitis pigmentosa mutants cause defects in the transition between the catalytic steps of splicing. Mayerle M, Guthrie C. RNA. 2016 May;22(5):793-809. doi: 10.1261/rna.055459.115. Epub 2016 Mar 11. 10.1261/rna.055459.115 PubMed 26968627
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