pRK7-myr-RSK1
(Plasmid
#8997)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8997 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRK7
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRSK
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SpeciesG. gallus (chicken)
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Entrez GeneRPS6KA1 (a.k.a. RPS6KA, RSK)
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Tag
/ Fusion Protein
- myr (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Src myristylation sequence (MGSSKSKPK) lacks the polybasic domain necessary for predominant membrane localization.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRK7-myr-RSK1 was a gift from John Blenis (Addgene plasmid # 8997 ; http://n2t.net/addgene:8997 ; RRID:Addgene_8997) -
For your References section:
Characterization of regulatory events associated with membrane targeting of p90 ribosomal S6 kinase 1. Richards SA, Dreisbach VC, Murphy LO, Blenis J. Mol Cell Biol. 2001 Nov . 21(21):7470-80. 10.1128/MCB.21.21.7470-7480.2001 PubMed 11585927
Map uploaded by the depositor.
