pRS313-PRP8
(Plasmid
#89966)
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Purposecomplements prp8 genomic deletion
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 89966 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS313
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Vector typeYeast Expression
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS313-PRP8 was a gift from Christine Guthrie (Addgene plasmid # 89966 ; http://n2t.net/addgene:89966 ; RRID:Addgene_89966) -
For your References section:
Prp8 retinitis pigmentosa mutants cause defects in the transition between the catalytic steps of splicing. Mayerle M, Guthrie C. RNA. 2016 May;22(5):793-809. doi: 10.1261/rna.055459.115. Epub 2016 Mar 11. 10.1261/rna.055459.115 PubMed 26968627