pRK7-FLAG-TSC2
(Plasmid #8996)

• Depositing Lab: John Blenis
• Publication: Tee et al Proc Natl Acad Sci U S A. 2002 Oct 15. 99(21):13571-6.

Backbone

• Vector backbone: pRK7
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TSC2
• Alt name: tuberin
• Species: H. sapiens (human)
• Insert Size (bp): 5400
• Entrez Gene: TSC2 (a.k.a. LAM, PPP1R160, TSC4)
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry