pRK7-FLAG-TSC1
(Plasmid #8995)

• Depositing Lab: John Blenis
• Publication: Tee et al Proc Natl Acad Sci U S A. 2002 Oct 15. 99(21):13571-6.

Backbone

• Vector backbone: pRK7
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TSC1
• Alt name: hamartin
• Species: H. sapiens (human)
• Insert Size (bp): 3500
• Entrez Gene: TSC1 (a.k.a. LAM, TSC)
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: N/A (unknown if destroyed)
• 3′ cloning site: N/A (unknown if destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: SV40pA-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene sequencing results find amino acid substitutions R768H and I812M compared to hamartin reference sequence NP_000359.1.