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pMRXIP-GFP-STX17ΔNTD
(Plasmid #89941)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89941 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMRXIP GFP-Ci2
  • Backbone manufacturer
    Dr.Shoji Yamaoka of Tokyo Medical and Dental University
  • Backbone size w/o insert (bp) 6875
  • Total vector size (bp) 7308
  • Vector type
    Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human syntaxin17 lacking N-terminal domain
  • Alt name
    STX17ΔNTD
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    433
  • Mutation
    deleted amino acids 1-161
  • GenBank ID
    NM_017919
  • Entrez Gene
    STX17
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer accggatccgATTCCTCAAGATCAAAATGCTGC
  • 3′ sequencing primer accggatccTTAACTGCATTTCTTGTCAG
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    It has the pMX backbone

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMRXIP-GFP-STX17ΔNTD was a gift from Noboru Mizushima (Addgene plasmid # 89941 ; http://n2t.net/addgene:89941 ; RRID:Addgene_89941)

  • For your References section:

    Accumulation of undegraded autophagosomes by expression of dominant-negative STX17 (syntaxin 17) mutants. Uematsu M, Nishimura T, Sakamaki Y, Yamamoto H, Mizushima N. Autophagy. 2017 Aug 3;13(8):1452-1464. doi: 10.1080/15548627.2017.1327940. Epub 2017 Jun 9. 10.1080/15548627.2017.1327940 PubMed 28598244
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