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pSUPER-retro-puro-shOrai1
(Plasmid #89933)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89933 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSUPER-puro
  • Backbone manufacturer
    oligoengine
  • Backbone size w/o insert (bp) 4400
  • Total vector size (bp) 4400
  • Vector type
    Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Orai1 shRNA
  • gRNA/shRNA sequence
    TCACTGGTTAGCCATAAGA
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_032790.3
  • Entrez Gene
    ORAI1 (a.k.a. CRACM1, IMD9, ORAT1, TAM2, TMEM142A)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSUPER-retro-puro-shOrai1 was a gift from Shengyu Yang (Addgene plasmid # 89933 ; http://n2t.net/addgene:89933 ; RRID:Addgene_89933)

  • For your References section:

    STIM1- and Orai1-mediated Ca(2+) oscillation orchestrates invadopodium formation and melanoma invasion. Sun J, Lu F, He H, Shen J, Messina J, Mathew R, Wang D, Sarnaik AA, Chang WC, Kim M, Cheng H, Yang S. J Cell Biol. 2014 Nov 24;207(4):535-48. doi: 10.1083/jcb.201407082. Epub 2014 Nov 17. 10.1083/jcb.201407082 PubMed 25404747
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