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Addgene

Profiline
(Plasmid #89925)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89925 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMW172
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use BL21 DE3 star for protein production
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    profiline-1
  • Alt name
    Pfn1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    140
  • GenBank ID
  • Entrez Gene
    PFN1 (a.k.a. ALS18)
  • Tag / Fusion Protein
    • None

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Profiline was a gift from Antoine Jégou & Guillaume Romet-Lemonne (Addgene plasmid # 89925 ; http://n2t.net/addgene:89925 ; RRID:Addgene_89925)

  • For your References section:

    Individual actin filaments in a microfluidic flow reveal the mechanism of ATP hydrolysis and give insight into the properties of profilin. Jegou A, Niedermayer T, Orban J, Didry D, Lipowsky R, Carlier MF, Romet-Lemonne G. PLoS Biol. 2011 Sep;9(9):e1001161. doi: 10.1371/journal.pbio.1001161. Epub 2011 Sep 27. 10.1371/journal.pbio.1001161 PubMed 21980262
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