p16L1-GFP
(Plasmid
#89910)
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PurposeSelf-packages in TT-16L2 stable line. L1-only capsid propagation.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 89910 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonemany different ones
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Backbone manufacturerInvitrogen
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Vector typeUnspecified
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Bleocin (Zeocin), 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHPV16L1+ GFP
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Alt namephüLsf
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SpeciesH. sapiens (human)
- Promoter SV40 and hEF1alpha
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xba1 (unknown if destroyed)
- 3′ cloning site Pst1 (unknown if destroyed)
- 5′ sequencing primer AGGCTCATGG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p16L1-GFP was a gift from John Schiller (Addgene plasmid # 89910 ; http://n2t.net/addgene:89910 ; RRID:Addgene_89910) -
For your References section:
Maturation of papillomavirus capsids. Buck CB, Thompson CD, Pang YY, Lowy DR, Schiller JT. J Virol. 2005 Mar;79(5):2839-46. 10.1128/JVI.79.5.2839-2846.2005 PubMed 15709003