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Addgene

pRK7-HA-S6K1-F5A-E389-R3A
(Plasmid #8991)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8991 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK7
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    S6K1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1500
  • Mutation
    F5A, T389E, R3A (C-terminal RSPRR motif to ASPAA)
  • Entrez Gene
    Rps6kb1 (a.k.a. p70 S6K-alpha)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See Schalm et. al., 2005 JBC Vol. 280(12)11101-11106.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK7-HA-S6K1-F5A-E389-R3A was a gift from John Blenis (Addgene plasmid # 8991 ; http://n2t.net/addgene:8991 ; RRID:Addgene_8991)

  • For your References section:

    Identification of a conserved motif required for mTOR signaling. Schalm SS, Blenis J. Curr Biol. 2002 Apr 16. 12(8):632-9. 10.1016/S0960-9822(02)00762-5 PubMed 11967149
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