pPbcas13b
(Plasmid #89906)

• Purpose: Bacterial expression for pbCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.
• Depositing Lab: Feng Zhang
• Publication: Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7. doi: 10.1016/j.molcel.2016.12.023. Epub 2017 Jan 5.

Backbone

• Vector backbone: pACYC184
• Backbone size w/o insert (bp): 4245
• Total vector size (bp): 7808
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Cas13b
• Species: P. buccae ATCC 33574
• Insert Size (bp): 3384
• Promoter: Lac

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: atgccggtactgccgggcctcttgcgggat

Resource Information

• Supplemental Documents:
  • ppbcas13b.gb
• A portion of this plasmid was derived from a plasmid made by: Cloned from genomic DNA purchased from ATCC of P. buccae ATCC 33574.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Benchling sequence link: https://benchling.com/s/hqikFD6s