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pcDNA3-FLAG-mTET2 CD mutant
(Plasmid #89896)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89896 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TET2
  • Alt name
    Tet Methylcytosine Dioxygenase 2
  • Alt name
    KIAA1546
  • Alt name
    Methylcytosine Dioxygenase TET2
  • Species
    M. musculus (mouse)
  • Mutation
    CD (cysteine-rich, dioxygenase domain) truncation of mouse TET2 (contains aa's 1044-1921); H1304Y, D1306A
  • GenBank ID
    NM_001040400.2 NM_001346736.1
  • Entrez Gene
    Tet2 (a.k.a. Ayu17-449, E130014J05Rik, mKIAA1546)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-FLAG-mTET2 CD mutant was a gift from Yue Xiong (Addgene plasmid # 89896 ; http://n2t.net/addgene:89896 ; RRID:Addgene_89896)

  • For your References section:

    CRL4(VprBP) E3 Ligase Promotes Monoubiquitylation and Chromatin Binding of TET Dioxygenases. Nakagawa T, Lv L, Nakagawa M, Yu Y, Yu C, D'Alessio AC, Nakayama K, Fan HY, Chen X, Xiong Y. Mol Cell. 2015 Jan 22;57(2):247-60. doi: 10.1016/j.molcel.2014.12.002. Epub 2014 Dec 31. 10.1016/j.molcel.2014.12.002 PubMed 25557551
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