pCrysβ:ECFP-LexOP:mCherry-HrasG12V
(Plasmid #89887)

• Purpose: Human HRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.
• Depositing Lab: Tatjana Sauka-Spengler
• Publication: Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: dmm.030056. doi: 10.1242/dmm.030056.

Backbone

• Vector backbone: pDs(cry:C-LOP:Ch)
• Backbone size w/o insert (bp): 6260
• Total vector size (bp): 7588
• Vector type: Unspecified

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry-HRasG12V
• Species: H. sapiens (human)
• Insert Size (bp): 1328
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CTCGACTCTAGGATCTTCGCA
• 3′ sequencing primer: GGGAGGTGTGGGAGGTTTT

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pDs(cry:ECFP-LexOP:Cherry) construct was from Dr Alexander Emelyanov and Dr Sergey Parinov pEFm.6 HA-KRasv12 was from Prof Xin Lu

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry