pCMV-bArrestin2-TevC-P2A-TdTomato
(Plasmid #89869)

• Purpose: expresses iTango bArrestin2-TevC component and TdTomato bicistronically
• Depositing Lab: Hyungbae Kwon
• Publication: Lee et al Nat Methods. 2017 May;14(5):495-503. doi: 10.1038/nmeth.4234. Epub 2017 Apr 3.

Backbone

• Vector backbone: pSyc181 P2A empty vector
• Total vector size (bp): 7121
• Vector type: Mammalian Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: bArrestin2-TevC-P2A-TdTomato
• Species: Synthetic
• Insert Size (bp): 3096
• Mutation: R173H in TdTomato (please see depositor comments below)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: M13 Reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that mutation R173H in TdTomato was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.