pCINeoFlag ALIX; I318A
(Plasmid #89867)

• Purpose: Expresses full length Flag-tagged ALIX with mutation I318A in mammalian cells; internal ID WISP11-403
• Depositing Lab: Wesley Sundquist
• Publication: Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.1371/journal.pone.0027466. Epub 2011 Dec 5.

Backbone

• Vector backbone: pcINeo
• Backbone manufacturer: Promega
• Total vector size (bp): 8109
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ALIX
• Alt name: AIP1
• Alt name: PDCD6IP
• Alt name: DRIP4
• Species: H. sapiens (human)
• Insert Size (bp): 2607
• Mutation: Mutated isoleucine 318 to alanine
• GenBank ID: 10015
• Entrez Gene: PDCD6IP (a.k.a. AIP1, ALIX, DRIP4, HP95, MCPH29)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nde1 (not destroyed)
• 3′ cloning site: BamH1 (not destroyed)
• 5′ sequencing primer: CMV for
• 3′ sequencing primer: CMV rev

Resource Information

• Supplemental Documents:
  • pCINeoFLAGALIX_I318A_WISP11-403.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry