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Addgene

pet151D-TOPO-BROX 1-411; L212D
(Plasmid #89864)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89864 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pet151D-TOPO
  • Backbone manufacturer
    Invitrogen
  • Total vector size (bp) 6996
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BROX
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1236
  • Mutation
    mutated leucine 212 to aspartic acid
  • Entrez Gene
    BROX (a.k.a. C1orf58)
  • Promoter T7
  • Tag / Fusion Protein
    • His Tag, V5 tag (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pet151D-TOPO-BROX 1-411; L212D was a gift from Wesley Sundquist (Addgene plasmid # 89864 ; http://n2t.net/addgene:89864 ; RRID:Addgene_89864)

  • For your References section:

    Structure of the Bro1 domain protein BROX and functional analyses of the ALIX Bro1 domain in HIV-1 budding. Zhai Q, Landesman MB, Robinson H, Sundquist WI, Hill CP. PLoS One. 2011;6(12):e27466. doi: 10.1371/journal.pone.0027466. Epub 2011 Dec 5. 10.1371/journal.pone.0027466 PubMed 22162750
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