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Addgene

pSUPER-retro-puro-Smad3
(Plasmid #89828)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89828 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSUPER-puro
  • Backbone manufacturer
    oligoengine
  • Backbone size w/o insert (bp) 4400
  • Total vector size (bp) 4400
  • Vector type
    Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SMAD3 shRNA
  • gRNA/shRNA sequence
    GGACGAGGTCTGCGTGAAT
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_001145102.1
  • Entrez Gene
    SMAD3 (a.k.a. HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3, hMAD-3, hSMAD3, mad3)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSUPER-retro-puro-Smad3 was a gift from Shengyu Yang (Addgene plasmid # 89828 ; http://n2t.net/addgene:89828 ; RRID:Addgene_89828)

  • For your References section:

    Fascin protein is critical for transforming growth factor beta protein-induced invasion and filopodia formation in spindle-shaped tumor cells. Sun J, He H, Xiong Y, Lu S, Shen J, Cheng A, Chang WC, Hou MF, Lancaster JM, Kim M, Yang S. J Biol Chem. 2011 Nov 11;286(45):38865-75. doi: 10.1074/jbc.M111.270413. Epub 2011 Sep 13. 10.1074/jbc.M111.270413 PubMed 21914811
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