N1-moxDendra2
(Plasmid
#89792)
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Purpose(Empty Backbone) C-terminal fusion to moxDendra2
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 89792 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-N1
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Modifications to backbonemoxDendra2 inserted via AgeI/NotI restriction sites, removing EGFP
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Vector typeMammalian Expression
- Promoter CMV
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Tag
/ Fusion Protein
- moxDendra2 (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
N1-moxDendra2 was a gift from Erik Snapp (Addgene plasmid # 89792 ; http://n2t.net/addgene:89792 ; RRID:Addgene_89792) -
For your References section:
moxDendra2: an inert photoswitchable protein for oxidizing environments. Kaberniuk AA, Morano NC, Verkhusha VV, Snapp EL. Chem Commun (Camb). 2017 Feb 9;53(13):2106-2109. doi: 10.1039/c6cc09997a. 10.1039/c6cc09997a PubMed 28133646
