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Addgene

JM3 [JBEI-6891]
(Plasmid #89788)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89788 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBBR1-MCS2
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    E. coli S17-lambda pir
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    tesA (E. coli), fadB (E. coli), Mlut_11700 (M. luteus) and fadM (E. coli)

Cloning Information

  • Cloning method Unknown

Resource Information

  • Supplemental Documents

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Growth Strain: E. coli S17-lambda pir, Medium Copy, gene from E.Coli

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    JM3 [JBEI-6891] was a gift from Harry Beller (Addgene plasmid # 89788 ; http://n2t.net/addgene:89788 ; RRID:Addgene_89788)
  • For your References section:

    Engineering of Ralstonia eutropha H16 for autotrophic and heterotrophic production of methyl ketones. Muller J, MacEachran D, Burd H, Sathitsuksanoh N, Bi C, Yeh YC, Lee TS, Hillson NJ, Chhabra SR, Singer SW, Beller HR. Appl Environ Microbiol. 2013 Jul;79(14):4433-9. doi: 10.1128/AEM.00973-13. Epub 2013 May 17. 10.1128/AEM.00973-13 PubMed 23686271