JM3 [JBEI-6891]
(Plasmid
#89788)
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PurposeBroad host range vector pBBR1-MCS2 containing 'tesA (E. coli), fadB (E. coli), Mlut_11700 (M. luteus) and fadM (E. coli) under control of BAD promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 89788 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBBR1-MCS2
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)E. coli S17-lambda pir
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Copy numberUnknown
Gene/Insert
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Gene/Insert nametesA (E. coli), fadB (E. coli), Mlut_11700 (M. luteus) and fadM (E. coli)
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Growth Strain: E. coli S17-lambda pir, Medium Copy, gene from E.Coli
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
JM3 [JBEI-6891] was a gift from Harry Beller (Addgene plasmid # 89788 ; http://n2t.net/addgene:89788 ; RRID:Addgene_89788) -
For your References section:
Engineering of Ralstonia eutropha H16 for autotrophic and heterotrophic production of methyl ketones. Muller J, MacEachran D, Burd H, Sathitsuksanoh N, Bi C, Yeh YC, Lee TS, Hillson NJ, Chhabra SR, Singer SW, Beller HR. Appl Environ Microbiol. 2013 Jul;79(14):4433-9. doi: 10.1128/AEM.00973-13. Epub 2013 May 17. 10.1128/AEM.00973-13 PubMed 23686271