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Addgene

isoform PKC Alpha (JCE599)
(Plasmid #89779)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89779 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pProm-PKC1-Adh1t
  • Modifications to backbone
    pProm-PKC1-Adh1t is YCplac33 with yeast PKC1 promoter and Adh1t terminator
  • Vector type
    Mammalian Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PKC Alpha
  • Alt name
    protein kinase C, alpha
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Prkca (a.k.a. Pkca)
  • Promoter yeast PKC1 promoter (-600 to -1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer M13-F20
  • 3′ sequencing primer M13-Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    isoform PKC Alpha (JCE599) was a gift from Juan Carlos Igual (Addgene plasmid # 89779 ; http://n2t.net/addgene:89779 ; RRID:Addgene_89779)

  • For your References section:

    Protein kinase C controls activation of the DNA integrity checkpoint. Soriano-Carot M, Quilis I, Bano MC, Igual JC. Nucleic Acids Res. 2014 Jun;42(11):7084-95. doi: 10.1093/nar/gku373. Epub 2014 May 3. 10.1093/nar/gku373 PubMed 24792164
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