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Addgene

pPKC1 (JCE575)
(Plasmid #89773)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89773 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    YCplac33
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PKC1
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    PKC1 (a.k.a. YBL105C, CLY15, CLY5, CLY7, HPO2, RSF8, STT1)
  • Promoter yeast PKC

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sph1 (not destroyed)
  • 3′ cloning site Sph1 (not destroyed)
  • 5′ sequencing primer M13-F20
  • 3′ sequencing primer M13-Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPKC1 (JCE575) was a gift from Juan Carlos Igual (Addgene plasmid # 89773 ; http://n2t.net/addgene:89773 ; RRID:Addgene_89773)

  • For your References section:

    Protein kinase C controls activation of the DNA integrity checkpoint. Soriano-Carot M, Quilis I, Bano MC, Igual JC. Nucleic Acids Res. 2014 Jun;42(11):7084-95. doi: 10.1093/nar/gku373. Epub 2014 May 3. 10.1093/nar/gku373 PubMed 24792164
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