pLenti6-CENP-A-mCherry
(Plasmid #89767)

• Purpose: Lentivirus destination vector for stable expression of mCherry-tagged kinetochore marker CENP-A
• Depositing Lab: Torsten Wittmann
• Publication: Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412. doi: 10.1242/jcs.194662. Epub 2017 Feb 23.

Backbone

• Vector backbone: pLenti6/V5-DEST
• Backbone manufacturer: ThermoFisher
• Backbone size w/o insert (bp): 7000
• Total vector size (bp): 8300
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CENP-A
• Species: H. sapiens (human)
• Insert Size (bp): 1300
• Entrez Gene: CENPA (a.k.a. CENP-A, CenH3)
• Promoter: CMV
• Tags / Fusion Proteins:
  • 3xHA (C terminal on insert)
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: mCherry reverse

Resource Information

• Supplemental Documents:
  • pHP41_pLenti6_V5DEST_CENPA_mCherry.gbk
• A portion of this plasmid was derived from a plasmid made by: The original CENP-A clone was from Alexey Khodjakov.
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry