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PurposeQuantitative measurement of CArG response element activity.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 89762 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCSC-G
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert name6X CArG-RE
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Alt nameCArG-RE: CCATAAAAGG (sense)
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SpeciesSynthetic
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Tag
/ Fusion Protein
- CMV-ZsGreen (C terminal on backbone)
Resource Information
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
CArG response element inserted upstream upstream of the ZsGreen fluorescent reporter; also expresses DsRedExpress2
Information can also be found in Moharil, et al. PLoS One. 2015 Oct 27;10(10):e0141365. doi: 10.1371 https://www.ncbi.nlm.nih.gov/pubmed/26505747 .
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LVDP-CArG-RE-GPR was a gift from Stelios Andreadis (Addgene plasmid # 89762 ; http://n2t.net/addgene:89762 ; RRID:Addgene_89762) -
For your References section:
NANOG Reverses the Myogenic Differentiation Potential of Senescent Stem Cells by Restoring ACTIN Filamentous Organization and SRF-Dependent Gene Expression. Mistriotis P, Bajpai VK, Wang X, Rong N, Shahini A, Asmani M, Liang MS, Wang J, Lei P, Liu S, Zhao R, Andreadis ST. Stem Cells. 2017 Jan;35(1):207-221. doi: 10.1002/stem.2452. Epub 2016 Jul 11. 10.1002/stem.2452 PubMed 27350449