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Addgene

Sph1-VC
(Plasmid #89756)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89756 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 4699
  • Total vector size (bp) 8444
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SNCAIP
  • Alt name
    SYPH1, Sph1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3745
  • GenBank ID
    NM_005460
  • Entrez Gene
    SNCAIP (a.k.a. SYPH1, Sph1)
  • Promoter CMV
  • Tag / Fusion Protein
    • C-terminal part of Venus (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AflII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer bGH
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Original clone with the C-terminal part of Venus (alpha-Synuclein-VC) comes from Pamela J.McLean.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.03.05.583522 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Sph1-VC was a gift from Tiago Outeiro (Addgene plasmid # 89756 ; http://n2t.net/addgene:89756 ; RRID:Addgene_89756)

  • For your References section:

    Synphilin-1 as a modulator of aSyn assembly. Lázaro DF, Amen T, Gerhardt E, Santos PI, Milovanovic D, Höglinger G, Outeiro TF. bioRxiv 2024.03.05.583522 10.1101/2024.03.05.583522
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