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Addgene

pU6-Cj-sgRNA
(Plasmid #89753)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89753 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57
  • Backbone manufacturer
    genescript
  • Backbone size w/o insert (bp) 2710
  • Total vector size (bp) 3112
  • Vector type
    sgRNA expression under U6 promoter

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cj-SgRNA
  • gRNA/shRNA sequence
    sequence which will be cloned
  • Species
    compylobacter Jejuni
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NsiI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer gtaaaacgacggccagt
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pU6-Cj-sgRNA was a gift from Seokjoong Kim (Addgene plasmid # 89753 ; http://n2t.net/addgene:89753 ; RRID:Addgene_89753)

  • For your References section:

    In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni. Kim E, Koo T, Park SW, Kim D, Kim K, Cho HY, Song DW, Lee KJ, Jung MH, Kim S, Kim JH, Kim JH, Kim JS. Nat Commun. 2017 Feb 21;8:14500. doi: 10.1038/ncomms14500. 10.1038/ncomms14500 PubMed 28220790
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