pcDNA3-HA-ERK2 L198A
(Plasmid
#8975)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8975 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameERK2
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SpeciesR. norvegicus (rat)
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Insert Size (bp)1200
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MutationL198A
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Entrez GeneMapk1 (a.k.a. ERK-2, ERT1, Erk2, p42-MAPK)
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
DEF-domain docking mutant.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-HA-ERK2 L198A was a gift from John Blenis (Addgene plasmid # 8975 ; http://n2t.net/addgene:8975 ; RRID:Addgene_8975) -
For your References section:
Spatially separate docking sites on ERK2 regulate distinct signaling events in vivo. Dimitri CA, Dowdle W, MacKeigan JP, Blenis J, Murphy LO. Curr Biol. 2005 Jul 26. 15(14):1319-24. 10.1016/j.cub.2005.06.037 PubMed 16051177