pYpet-Optop38i
(Plasmid #89749)

• Purpose: Expression of light-regulated p38 inhibitor, Ypet-fused, wild-type photosensor, inhibitor MK3BD3-13F
• Depositing Lab: Michael Courtney
• Publication: Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10.1038/ncomms15017.

Backbone

• Vector backbone: pYpet-C1
• Backbone manufacturer: Derived by our lab using pExFP-C1 and JNKAREV
• Backbone size w/o insert (bp): 4731
• Total vector size (bp): 5180
• Vector type: Mammalian Expression ; Fluorescent protein
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Optop38i3
• Alt name: AsLov2Jalpha (404-546)-3-13F
• Species: Synthetic; Avena Sativa and
• Insert Size (bp): 481
• GenBank ID: KY595946
• Promoter: CMV
• Tag / Fusion Protein:
  • Ypet (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: YFP_Venus-fwd
• 3′ sequencing primer: fastbac reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: We designed and assembled the insert from de novo synthesised sequences
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please see
Melero-Fernandez de Mera RM*, Li LL*, Popinigis A, Cisek K, Tuittila M, Yadav L, Serva A, Courtney MJ (2017) A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs. Nat. Commun. 8, 15017 doi: 10.1038/ncomms15017 in press.