pKCAG-H2BmCherry-OptoJNKi
(Plasmid #89741)

• Purpose: Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to chromatin with H2B, wild-type photosensor, inhibitor JIP11
• Depositing Lab: Michael Courtney
• Publication: Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10.1038/ncomms15017.

Backbone

• Vector backbone: pmCherry-OptoJNKi
• Backbone manufacturer: Derived by our lab from pLuc-OptoJNKi and Cherry sequence
• Backbone size w/o insert (bp): 5240
• Total vector size (bp): 5680
• Vector type: Mammalian Expression ; Optogenetics
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: OptoJNKi
• Alt name: AsLov2Jalpha (404-546)-JIP11
• Species: Synthetic; Avena Sativa and
• Insert Size (bp): 478
• GenBank ID: KY595943
• Promoter: CAG
• Tags / Fusion Proteins:
  • mCherry (N terminal on backbone)
  • Histone2B (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: DsRed_mCherry-fwd
• 3′ sequencing primer: fastbac reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: We designed and assembled the insert from de novo synthesised sequences

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For further information please see:
Melero-Fernandez de Mera RM*, Li LL*, Popinigis A, Cisek K, Tuittila M, Yadav L, Serva A, Courtney MJ (2017) A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs. Nat. Commun. 8, 15017 doi: 10.1038/ncomms15017.