T7-AglK-His
(Plasmid #89714)

• Purpose: Expresses AglK from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.
• Depositing Lab: Barbara Imperiali
• Publication: Zamora et al Methods Enzymol. 2017;597:145-186. doi: 10.1016/bs.mie.2017.06.003. Epub 2017 Jul 5.

Backbone

• Vector backbone: pET24a(+)
• Backbone manufacturer: EMD Biosciences
• Backbone size w/o insert (bp): 5310
• Total vector size (bp): 6066
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: AglK
• Species: Methanococcus voltae
• Insert Size (bp): 756
• GenBank ID: EU726230.1
• Tags / Fusion Proteins:
  • T7 (N terminal on backbone)
  • His6 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: ATGGCTGATAAATTGATATATTTAATAATTCCCGCTTACAACGA
• 3′ sequencing primer: TTAATCCAAAACTTTGCCCATTTTTAACATAAGTAACC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry