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Addgene

GST-PglF130
(Plasmid #89708)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89708 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX4T-2
  • Backbone manufacturer
    GE Healthcare Life Sciences
  • Backbone size w/o insert (bp) 4900
  • Total vector size (bp) 6300
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pglF
  • Species
    Campylobacter jejuni
  • Insert Size (bp)
    1386
  • Mutation
    residues 1-129 of putative transmembrane domain have been deleted
  • GenBank ID
    905411
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer CGCGGATCCATGCTTGTGGATTTTAAACCTTC
  • 3′ sequencing primer CGGCTCGAGTCAGTGATGATGATGATGATGATGGTGTACACCTTCTTTATTGTGTTTAAATTC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-PglF130 was a gift from Barbara Imperiali (Addgene plasmid # 89708 ; http://n2t.net/addgene:89708 ; RRID:Addgene_89708)

  • For your References section:

    Chemoenzymatic Synthesis and Applications of Prokaryote-Specific UDP-Sugars. Zamora CY, Schocker NS, Chang MM, Imperiali B. Methods Enzymol. 2017;597:145-186. doi: 10.1016/bs.mie.2017.06.003. Epub 2017 Jul 5. 10.1016/bs.mie.2017.06.003 PubMed 28935101
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