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Addgene

pCMV-ZFN-AAVS1
(Plasmid #89707)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89707 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMVpA
  • Total vector size (bp) 5772
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ZFN-AAVS1
  • Insert Size (bp)
    2127
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (unknown if destroyed)
  • 3′ cloning site Acc65I (unknown if destroyed)
  • 5′ sequencing primer GACGCAAATGGGCGGTAG
  • 3′ sequencing primer CTATTGCTTTATTTGTAACCATTATAAGCTGC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-ZFN-AAVS1 was a gift from Dmitriy Mazurov (Addgene plasmid # 89707 ; http://n2t.net/addgene:89707 ; RRID:Addgene_89707)

  • For your References section:

    Gene Editing in Human Lymphoid Cells: Role for Donor DNA, Type of Genomic Nuclease and Cell Selection Method. Zotova A, Lopatukhina E, Filatov A, Khaitov M, Mazurov D. Viruses. 2017 Nov 2;9(11). pii: v9110325. doi: 10.3390/v9110325. 10.3390/v9110325 PubMed 29099045
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