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Addgene

pITRSF4
(Plasmid #89699)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89699 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSF-1b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3669
  • Modifications to backbone
    NcoI to NdeI mutation
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SMT3_GLP_H6_GFP_TRNGL
  • Insert Size (bp)
    1038
  • Promoter T7
  • Tag / Fusion Protein
    • SMT3 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pITRSF4 was a gift from Hideo Iwai (Addgene plasmid # 89699 ; http://n2t.net/addgene:89699 ; RRID:Addgene_89699)

  • For your References section:

    Segmental isotopic labeling of a single-domain globular protein without any refolding step by an asparaginyl endopeptidase. Mikula KM, Tascon I, Tommila JJ, Iwai H. FEBS Lett. 2017 Mar 30. doi: 10.1002/1873-3468.12640. 10.1002/1873-3468.12640 PubMed 28369872
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