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p426-SNR52p-gRNA.clr-2.Y-SUP4t
(Plasmid #89692)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89692 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS426
  • Backbone manufacturer
    ATCC
  • Backbone size w/o insert (bp) 5726
  • Total vector size (bp) 6274
  • Modifications to backbone
    The target sequence to csr-1 locus was added instead of CAN1 of p426-SNR52p-gRNA.CAN1.Y-SUP4t (Plasmid #43803) from Dr. Church lab.
  • Vector type
    CRISPR ; gRNA
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    gRNA for clr-2 locus
  • Alt name
    gRNA
  • Alt name
    clr-2
  • gRNA/shRNA sequence
    GAGTGCCCTAGTCGGTGTGA
  • Species
    N. crassa
  • Tag / Fusion Protein
    • No

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer GGAGCTTCTTTGAAAAGATAATGTATG
  • 3′ sequencing primer GTTACATGCGTACACGCGTC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p426-SNR52p-gRNA.clr-2.Y-SUP4t was a gift from Christian Hong (Addgene plasmid # 89692 ; http://n2t.net/addgene:89692 ; RRID:Addgene_89692)

  • For your References section:

    Efficient gene editing in Neurospora crassa with CRISPR technology. Matsu-Ura T, Baek M, Kwon J, Hong C. Fungal Biol Biotechnol. 2015 Jul 15;2:4. doi: 10.1186/s40694-015-0015-1. eCollection 2015. 10.1186/s40694-015-0015-1 PubMed 28955455
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