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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8968 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namec-Fos
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Alt nameFos
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SpeciesR. norvegicus (rat)
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Insert Size (bp)1200
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MutationS362D, S374D
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Entrez GeneFos (a.k.a. c-fos)
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
ERK and RSK coordinately phosphorylate Ser374 and Ser362, respectively. Mutating these residues to Asp mimics phosphorylation.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-FLAG-Fos DD was a gift from John Blenis (Addgene plasmid # 8968 ; http://n2t.net/addgene:8968 ; RRID:Addgene_8968) -
For your References section:
Molecular interpretation of ERK signal duration by immediate early gene products. Murphy LO, Smith S, Chen RH, Fingar DC, Blenis J. Nat Cell Biol. 2002 Aug . 4(8):556-64. 10.1038/ncb822 PubMed 12134156