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Addgene

pcDNA3-FLAG-Fos AA
(Plasmid #8967)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8967 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    c-Fos
  • Alt name
    Fos
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1200
  • Mutation
    S362A, S374A
  • Entrez Gene
    Fos (a.k.a. c-fos)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

ERK and RSK coordinately phosphorylate Ser374 and Ser362, respectively.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-FLAG-Fos AA was a gift from John Blenis (Addgene plasmid # 8967 ; http://n2t.net/addgene:8967 ; RRID:Addgene_8967)
  • For your References section:

    Molecular interpretation of ERK signal duration by immediate early gene products. Murphy LO, Smith S, Chen RH, Fingar DC, Blenis J. Nat Cell Biol. 2002 Aug . 4(8):556-64. 10.1038/ncb822 PubMed 12134156