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Addgene

pcDNA3-FLAG-Fos WT
(Plasmid #8966)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8966 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    c-Fos
  • Alt name
    Fos
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1200
  • Entrez Gene
    Fos (a.k.a. c-fos)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-FLAG-Fos WT was a gift from John Blenis (Addgene plasmid # 8966 ; http://n2t.net/addgene:8966 ; RRID:Addgene_8966)

  • For your References section:

    Molecular interpretation of ERK signal duration by immediate early gene products. Murphy LO, Smith S, Chen RH, Fingar DC, Blenis J. Nat Cell Biol. 2002 Aug . 4(8):556-64. 10.1038/ncb822 PubMed 12134156
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