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Addgene

Lenti-sgNeo3/Cre
(Plasmid #89653)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89653 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLL3.3
  • Backbone size w/o insert (bp) 7741
  • Vector type
    Mammalian Expression, Lentiviral, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gRNA targeting Neomycin
  • gRNA/shRNA sequence
    GAATAGCCTCTCCACCCAAG
  • Species
    M. musculus (mouse)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that mutation R24S in Cre-recombinase was found during Addgene's quality control. The depositor noted that this mutation does NOT affect generating functional virus.

Annotated sequence at https://benchling.com/s/seq-gn6vrVDEVFP1u59whOCA

To view Winslow Lab vector sequences on Benchling, please visit https://benchling.com/winslowlab/f_/rgXCLH0mq9-winslow-lab-public-vectors-and-sequences/?sort=name

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lenti-sgNeo3/Cre was a gift from Monte Winslow (Addgene plasmid # 89653 ; http://n2t.net/addgene:89653 ; RRID:Addgene_89653)

  • For your References section:

    A quantitative and multiplexed approach to uncover the fitness landscape of tumor suppression in vivo. Rogers ZN, McFarland CD, Winters IP, Naranjo S, Chuang CH, Petrov D, Winslow MM. Nat Methods. 2017 May 22. doi: 10.1038/nmeth.4297. 10.1038/nmeth.4297 PubMed 28530655
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