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PurposeExpresses a p53-targeting gRNA and Cre-recombinase
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 89646 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLL3.3
- Backbone size w/o insert (bp) 7741
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Vector typeMammalian Expression, Lentiviral, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namegRNA targeting p53
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gRNA/shRNA sequenceAGGAGCTCCTGACACTCGGA
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SpeciesM. musculus (mouse)
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Entrez GeneTrp53 (a.k.a. Tp53, bbl, bfy, bhy, p44, p53)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Annotated sequence at https://benchling.com/s/seq-8mziNm8Nq2jL95vLxgTJ
To view Winslow Lab vector sequences on Benchling, please visit https://benchling.com/winslowlab/f_/rgXCLH0mq9-winslow-lab-public-vectors-and-sequences/?sort=name
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Lenti-sgp53/Cre was a gift from Monte Winslow (Addgene plasmid # 89646 ; http://n2t.net/addgene:89646 ; RRID:Addgene_89646) -
For your References section:
A quantitative and multiplexed approach to uncover the fitness landscape of tumor suppression in vivo. Rogers ZN, McFarland CD, Winters IP, Naranjo S, Chuang CH, Petrov D, Winslow MM. Nat Methods. 2017 May 22. doi: 10.1038/nmeth.4297. 10.1038/nmeth.4297 PubMed 28530655
