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Addgene

pcDNA3.1-dCas9-MQ1(S317A)-EGFP
(Plasmid #89636)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89636 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    invitrogene
  • Backbone size w/o insert (bp) 5382
  • Total vector size (bp) 11637
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Neomycin (select with G418) ; EGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    site-specific DNA-methyltransferase SssI
  • Alt name
    dCas9-MQ1(S317A)
  • Species
    Synthetic; Spiroplasma sp. (strain MQ1)
  • Insert Size (bp)
    1164
  • Mutation
    S317A, AGC-->GCC
  • GenBank ID
    P15840.3
  • Promoter CMV
  • Tags / Fusion Proteins
    • 3*FLAG (N terminal on insert)
    • 6*His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (destroyed during cloning)
  • 3′ cloning site NheI (destroyed during cloning)
  • 5′ sequencing primer GCGCCCTAGGGACAGCAAAGTGGAGAACAAAACA
  • 3′ sequencing primer GCGCCCTAGGGCCGCCGATCTTGTCAATG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-dCas9-MQ1(S317A)-EGFP was a gift from Margaret Goodell (Addgene plasmid # 89636 ; http://n2t.net/addgene:89636 ; RRID:Addgene_89636)

  • For your References section:

    Targeted DNA methylation in vivo using an engineered dCas9-MQ1 fusion protein. Lei Y, Zhang X, Su J, Jeong M, Gundry MC, Huang YH, Zhou Y, Li W, Goodell MA. Nat Commun. 2017 Jul 11;8:16026. doi: 10.1038/ncomms16026. 10.1038/ncomms16026 PubMed 28695892
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