Rc/CMV cyclin E
(Plasmid #8963)

• Depositing Lab: Bob Weinberg
• Publication: Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

Backbone

• Vector backbone: pRc/CMV
• Backbone size w/o insert (bp): 5542
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cyclin E
• Species: H. sapiens (human)
• Insert Size (bp): 2500
• GenBank ID: NM_001322262.1
• Entrez Gene: CCNE1 (a.k.a. CCNE, pCCNE1)
• Promoter: CMV
• Tag / Fusion Protein:
  • GPI linker (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BstXI (destroyed during cloning)
• 3′ cloning site: BstXI (destroyed during cloning)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Addgene Notes:
  • Further sequence from requesting scientist
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See author's map. Please note BstXI was used to clone in insert (blunt ligation). There are also 2 EcoRI sites flanking the insert.