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Addgene

pET21a - TryX
(Plasmid #89623)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89623 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tryparedoxin
  • Alt name
    TyrX
  • Species
    T. cruzi
  • Insert Size (bp)
    432
  • GenBank ID
    Tc00.1047053509997.30 Tc00.1047053509997.30
  • Tags / Fusion Proteins
    • 6HIS (C terminal on insert)
    • T7 tag (gene 10 leader) (N terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS results found K13N and A95T within the Tryparedoxin translation compared to Tc00.1047053509997.30. Depositor states that mutation is outside of functional domains

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21a - TryX was a gift from Judy Lieberman (Addgene plasmid # 89623 ; http://n2t.net/addgene:89623 ; RRID:Addgene_89623)

  • For your References section:

    Killer lymphocytes use granulysin, perforin and granzymes to kill intracellular parasites. Dotiwala F, Mulik S, Polidoro RB, Ansara JA, Burleigh BA, Walch M, Gazzinelli RT, Lieberman J. Nat Med. 2016 Feb;22(2):210-6. doi: 10.1038/nm.4023. Epub 2016 Jan 11. 10.1038/nm.4023 PubMed 26752517
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