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pCCLc-MNDU3-VEGFA-PGK-EGFP-WPRE
(Plasmid #89609)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89609 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CCLc
  • Backbone manufacturer
    Luigi Naldini, PMID 9765382
  • Backbone size w/o insert (bp) 8499
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    EGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    VEGF-A(165)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    423
  • Mutation
    K188E
  • GenBank ID
    NM_001025366.1
  • Entrez Gene
    VEGFA (a.k.a. L-VEGF, MVCD1, VEGF, VPF)
  • Promoter MNDU3

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCCLc-MNDU3-VEGFA-PGK-EGFP-WPRE was a gift from Fernando Fierro (Addgene plasmid # 89609 ; http://n2t.net/addgene:89609 ; RRID:Addgene_89609)
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