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pCCLc-U6-shHMGA2.2-PGK-dTomato
(Plasmid #89605)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89605 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CCLc
  • Backbone manufacturer
    Luigi Naldini, PMID 9765382
  • Backbone size w/o insert (bp) 8661
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    dTomato

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    shRNA targeting HMGA2
  • gRNA/shRNA sequence
    AAAAAAGGGAAGAGGAGGAGGAATTTTTCTCTTGGTAAAATTCCTCC TCCTCTTCCCGGTGTTTCGTCCTTTCCACAAG
  • Species
    H. sapiens (human)
  • Entrez Gene
    HMGA2 (a.k.a. BABL, HMGI-C, HMGIC, LIPO, SRS5, STQTL9)
  • Promoter U6

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCCLc-U6-shHMGA2.2-PGK-dTomato was a gift from Fernando Fierro (Addgene plasmid # 89605 ; http://n2t.net/addgene:89605 ; RRID:Addgene_89605)

  • For your References section:

    Fibroblast Growth Factor 2 Regulates High Mobility Group A2 Expression in Human Bone Marrow-Derived Mesenchymal Stem Cells. Kalomoiris S, Cicchetto AC, Lakatos K, Nolta JA, Fierro FA. J Cell Biochem. 2016 Sep;117(9):2128-37. doi: 10.1002/jcb.25519. Epub 2016 Mar 8. 10.1002/jcb.25519 PubMed 26888666
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